Monoclonal Anti-EDG1 antibody produced in mouse
SIGMA/WH0001901M1 - clone 2E12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CHEDG1; Anti-D1S3362; Anti-ECGF1; Anti-EDG1; Anti-S1PR1; Anti-endothelial differentiation, sphingolipid G-protein-coupled receptor, 1
MDL Number: MFCD02264095
Product Type: Chemical
Western Blotting EDG1 monoclonal antibody, clone 2E12 Western Blot analysis of EDG1 expression in Jurkat.
Western Blotting Western Blot analysis of EDG1 expression in transfected 293T cell line by EDG1 monoclonal antibody, clone 2E12. Lanes Lane 1: EDG1 transfected lysate (42.8 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (30.91 kDa).
ELISA Detection limit for recombinant GST tagged EDG1 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC018650   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P21453 |
| General description: | The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. (provided by RefSeq) |
| Immunogen: | EDG1 (AAH18650, 1 a.a. ~ 47 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGPTSVPLVKAHRSSVSDYVNYDII |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |