Monoclonal Anti-EDN3 antibody produced in mouse
SIGMA/WH0001908M1 - clone 2A6-2A4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ET3; Anti-MGC15067; Anti-MGC61498; Anti-endothelin 3
MDL Number: MFCD00162281
Product Type: Chemical
Western Blotting EDN3 monoclonal antibody, clone 2A6-2A4 Western Blot analysis of EDN3 expression in LNCaP.
Western Blotting Western Blot analysis of EDN3 expression in transfected 293T cell line by EDN3 monoclonal antibody, clone 2A6-2A4. Lanes Lane 1: EDN3 transfected lysate (25.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (51.92 kDa).
Immunoprecipitation Immunoprecipitation of EDN3 transfected lysate using anti-EDN3 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with EDN3 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged EDN3 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2A6-2A4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC008876   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P14138 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Four alternatively spliced transcript variants encoding three distinct isoforms have been observed. (provided by RefSeq) |
| Immunogen: | EDN3 (AAH08876, 1 a.a. ~ 238 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MEPGLWLLFGLTVTSAAGFVPCSQS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |