Monoclonal Anti-EIF2S1 antibody produced in mouse
SIGMA/WH0001965M1 - clone 3H12-C11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-EIF2; Anti-EIF2A; Anti-EIF2alpha; Anti-eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to EIF2S1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting EIF2S1 monoclonal antibody, clone 3H12-C11. Western Blot analysis of EIF2S1 expression in HeLa S3 NE.
Enhanced Validation-RNAi Western blot analysis of EIF2S1 over-expressed 293 cell line, cotransfected with EIF2S1 Validated Chimera RNAi. Blot probed with EIF2S1 monoclonal antibody, clone 3H12-C11. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting EIF2S1 monoclonal antibody, clone 3H12-C11 Western Blot analysis of EIF2S1 expression in HeLa.
Western Blotting Western Blot analysis of EIF2S1 expression in transfected 293T cell line by EIF2S1 monoclonal antibody, clone 3H12-C11. Lanes Lane 1: EIF2S1 transfected lysate (36.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (60.39 kDa).
ELISA Detection limit for recombinant GST tagged EIF2S1 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3H12-C11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC002513   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P05198 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM |
| Immunogen: | EIF2S1 (AAH02513.1, 1 a.a. ~ 315 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MPGLSCRFYQHKFPEVEDVVMVNVR |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |