Monoclonal Anti-ELK1 antibody produced in mouse
SIGMA/WH0002002M1 - clone 2G6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ELK1, member of ETS oncogene family
MDL Number: MFCD01092331
Product Type: Chemical
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between MAPK3 and ELK1. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-ELK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
ELISA Detection limit for recombinant GST tagged ELK1 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2G6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_005229   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| proximity ligation assay: suitable | |
| UniProt accession no. | P19419 |
| General description: | This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene. (provided by RefSeq) |
| Immunogen: | ELK1 (NP_005220, 67 a.a. ~ 166 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence YYDKNIIRKVSGQKFVYKFVSYPEV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |