Monoclonal Anti-ENO1 antibody produced in mouse
SIGMA/WH0002023M1 - clone 8G8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ENO1L1; Anti-MBP1; Anti-MPB1; Anti-NNE; Anti-PPH; Anti-enolase 1, (alpha)
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to ENO1 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 1 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to ENO1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody, clone 8G8. Lanes Lane 1: ENO1 transfected lysate (47 kDa). Lane 2: Non-transfected lysate.
Western Blotting ENO1 monoclonal antibody, clone 8G8 Western Blot analysis of ENO1 expression in MCF-7.
Western Blotting ENO1 monoclonal antibody, clone 8G8. Western Blot analysis of ENO1 expression in human colon.
Western Blotting QC Western Blot detection against Immunogen (73.48 kDa).
Immunoprecipitation Immunoprecipitation of ENO1 transfected lysate using anti-ENO1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with ENO1 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged ENO1 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 8G8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC022545   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P06733 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. (provided by RefSeq) |
| Immunogen: | ENO1 (AAH22545, 1 a.a. ~ 434 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSILKIHAREIFDSRGNPTVEVDLF |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |