Monoclonal Anti-FAP antibody produced in mouse
SIGMA/WH0002191M1 - clone 1E5, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DPPIV; Anti-FAPA; Anti-SEPRASE; Anti-fibroblast activation protein, alpha
MDL Number: MFCD03791521
Product Type: Chemical
Western Blotting Western Blot analysis of FAP expression in transfected 293T cell line by FAP monoclonal antibody, clone 1E5. Lanes Lane 1: FAP transfected lysate (87.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting FAP monoclonal antibody, clone 1E5 Western Blot analysis of FAP expression in HepG2.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
Immunoprecipitation Immunoprecipitation of FAP transfected lysate using anti-FAP monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with FAP rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged FAP is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1E5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC026250   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q12884 |
| Application: | Monoclonal Anti-FAP antibody produced in mouse has been used in western blotting. |
| Biochem/physiol Actions: | Fibroblast activation protein (FAP) participates in the remodeling of tissue, wound healing, inflammation, fibrosis, and tumor development. It possesses dipeptidyl peptidase and endopeptidase activities. FAP plays a key role in the remodeling of extracellular matrix structure and the reconstruction of tumor microarray. Overexpression of the FAP gene might return epithelial ovarian cancer after chemotherapy. FAP gene expression is involved in cancer cells and premalignant metaplastic cells of the esophagus. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Fibroblast activation protein (FAP), a cell-surface type II transmembrane glycoprotein serine protease, has a cytoplasmic tail, a single transmembrane domain, and an extracellular domain. It is a member of the S9b family of post-proline cleaving enzymes. FAP is localized in the plasma membrane. FAP gene is mapped to human chromosome 2q24.2. Expression of FAP is hardly seen in adult tissues. |
| Immunogen: | FAP (AAH26250, 525 a.a. ~ 624 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PPQFDRSKKYPLLIQVYGGPCSQSV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |