Monoclonal Anti-FASN antibody produced in mouse
SIGMA/WH0002194M1 - clone 3F2-1F3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FAS; Anti-MGC14367; Anti-MGC15706; Anti-OA519; Anti-fatty acid synthase
MDL Number: MFCD02262888
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to FASN on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 10 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to FASN on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of FASN expression in transfected 293T cell line by FASN monoclonal antibody, clone 3F2-1F3. Lanes Lane 1: FASN transfected lysate (48.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting FASN monoclonal antibody, clone 3F2-1F3 Western Blot analysis of FASN expression in 293.
Western Blotting QC Western Blot detection against Immunogen (74.03 kDa).
Immunoprecipitation Immunoprecipitation of FASN transfected lysate using anti-FASN monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with FASN rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged FASN is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3F2-1F3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC007909   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P49327 |
| Biochem/physiol Actions: | FASN (fatty acid synthase) is essential for de novo fatty-acid synthesis. It plays a major role in glioma neovascularization. The product of this gene helps in the production of fatty acids from acetyl-CoA and malonyl-CoA. Absence of FASN may block migration, invasion and healing of hepatoma carcinoma cells by reducing HIF-1α (hypoxia-inducible factor 1-α) and IGFBP1 (insulin-like growth factor binding protein-1). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | FASN (fatty acid synthase) encodes a fatty acid synthase (FAS) enzyme. This gene is located on human chromosome 17. |
| General description: | The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. (provided by RefSeq) |
| Immunogen: | FASN (AAH07909, 1 a.a. ~ 439 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSTNDTIVSGTLPQRMASCLEVLDL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |