Monoclonal Anti-FCER1A antibody produced in mouse
SIGMA/WH0002205M1 - clone 2C12-3B6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FCE1A; Anti-Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; Anti-FcERI
Product Type: Chemical
Western Blotting Western Blot analysis of FCER1A expression in transfected 293T cell line by FCER1A monoclonal antibody, clone 2C12-3B6. Lanes Lane 1: FCER1A transfected lysate (29.6 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of FCER1A over-expressed 293 cell line, cotransfected with FCER1A Validated Chimera RNAi. Blot probed with FCER1A monoclonal antibody, clone 2C12-3B6. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting FCER1A monoclonal antibody, clone 2C12-3B6 Western Blot analysis of FCER1A expression in A-549.
Western Blotting QC Western Blot detection against Immunogen (54.01 kDa).
ELISA Detection limit for recombinant GST tagged FCER1A is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2C12-3B6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC005912   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P12319 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The IgE receptor plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more high-affinity IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. The IgE receptor consists of 3 subunits: alpha, beta (MIM 147138), and gamma (MIM 147139); only the alpha subunit is glycosylated.[supplied by OMIM |
| Immunogen: | FCER1A (AAH05912.1, 1 a.a. ~ 257 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAPAMESPTLLCVALLFFAPDGVLA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |