Monoclonal Anti-FGL1 antibody produced in mouse
SIGMA/WH0002267M1 - clone 2A4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HFREP1; Anti-HP041; Anti-LFIRE1; Anti-MGC12455; Anti-fibrinogen-like 1
Product Type: Chemical
Western Blotting Western Blot analysis of FGL1 expression in transfected 293T cell line by FGL1 monoclonal antibody, clone 2A4. Lanes Lane 1: FGL1 transfected lysate (36.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (57.97 kDa).
Immunoprecipitation Immunoprecipitation of FGL1 transfected lysate using anti-FGL1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with FGL1 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged FGL1 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2A4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC007047   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q08830 |
| General description: | Fibrinogen-like 1 is a member of the fibrinogen family. This protein is homologous to the carboxy terminus of the fibrinogen beta- and gamma- subunits which contains the four conserved cysteines of fibrinogens and fibrinogen related proteins. However, this protein lacks the platelet-binding site, cross-linking region and a thrombin-sensitive site which are necessary for fibrin clot formation. This protein may play a role in the development of hepatocellular carcinomas. Four alternatively spliced transcript variants encoding the same protein exist for this gene. (provided by RefSeq) |
| Immunogen: | FGL1 (AAH07047.1, 19 a.a. ~ 312 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence EISALEDCAQEQMRLRAQVRLLETR |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |