Monoclonal Anti-FKBP4 antibody produced in mouse
SIGMA/WH0002288M1 - clone 5C11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FK506 binding protein 4, 59kDa; Anti-FKBP52; Anti-FKBP59; Anti-HBI; Anti-Hsp56; Anti-PPIase; Anti-p52
MDL Number: MFCD01865536
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to FKBP4 on formalin-fixed paraffin-embedded human uterine cervix. [antibody concentration 0.5 μg/mL]
Western Blotting FKBP4 monoclonal antibody, clone 5C11. Western Blot analysis of FKBP4 expression in NIH/3T3.
Western Blotting FKBP4 monoclonal antibody, clone 5C11 Western Blot analysis of FKBP4 expression in HeLa.
Western Blotting FKBP4 monoclonal antibody, clone 5C11. Western Blot analysis of FKBP4 expression in PC-12.
Western Blotting FKBP4 monoclonal antibody, clone 5C11. Western Blot analysis of FKBP4 expression in Raw 264.7.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
Immunoprecipitation Immunoprecipitation of FKBP4 transfected lysate using anti-FKBP4 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with FKBP4 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged FKBP4 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5C11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC007924   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q02790 |
| General description: | The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. (provided by RefSeq) |
| Immunogen: | FKBP4 (AAH07924, 301 a.a. ~ 410 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence EYESSFSNEEAQKAQALRLASHLNL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |