Monoclonal Anti-GATA2 antibody produced in mouse
SIGMA/WH0002624M1 - clone 2D11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-GATA binding protein 2; Anti-MGC2306; Anti-NFE1B
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to GATA2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting GATA2 monoclonal antibody, clone 2D11 Western Blot analysis of GATA2 expression in HeLa S3 NE.
Western Blotting Western Blot analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody, clone 2D11. Lanes Lane 1: GATA2 transfected lysate (50.5 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi. Blot probed with GATA2 monoclonal antibody, clone 2D11. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (36.96 kDa).
ELISA Detection limit for recombinant GST tagged GATA2 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2D11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC018988   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P23769 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants |
| Immunogen: | GATA2 (AAH18988, 1 a.a. ~ 102 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MEVAPEQPRWMAHPAVLNAQHPDSH |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |