Monoclonal Anti-GBA antibody produced in mouse
SIGMA/WH0002629M1 - clone 2e2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-GBA1; Anti-GCB; Anti-GLUC; Anti-glucosidase, beta; acid (includes glucosylceramidase)
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to GBA on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to GBA on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of GBA expression in transfected 293T cell line by GBA monoclonal antibody, clone 2E2. Lanes Lane 1: GBA transfected lysate (60 kDa). Lane 2: Non-transfected lysate.
Western Blotting GBA monoclonal antibody, clone 2E2 Western Blot analysis of GBA expression in MCF-7.
Western Blotting QC Western Blot detection against Immunogen (35.64 kDa).
ELISA Detection limit for recombinant GST tagged GBA is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2e2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_000157   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P04062 |
| Application: | Monoclonal Anti-GBA antibody has been used in immunofluorescence staining. |
| Biochem/physiol Actions: | GBA (β-glucocerebrosidase) is responsible for the conversion of the glycolipid glucocerebroside to ceramide and glucose. Mutations in this gene are associated with Gaucher disease and Parkinson disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | GBA (β-glucocerebrosidase) gene is mapped to human chromosome 1q21. It is a lysosomal enzyme and is widely expressed. The protein has domain I with three‐stranded anti‐parallel β‐sheets, domain II with two β‐sheets making an immunoglobulin‐like domain and domain III with eight‐stranded β/αtriosephosphate isomerase (TIM) barrel. |
| General description: | This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants encoding the same protein. (provided by RefSeq) |
| Immunogen: | GBA (NP_000148, 146 a.a. ~ 235 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence SYFSEEGIGYNIIRVPMASCDFSIR |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |