Monoclonal Anti-GJB3 antibody produced in mouse
SIGMA/WH0002707M1 - clone 3B4-1B3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CX31; Anti-DFNA2; Anti-FLJ22486; Anti-MGC102938; Anti-gap junction protein, beta 3, 31kDa (connexin 31)
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to GJB3 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting GJB3 monoclonal antibody, clone 3B4-1B3 Western Blot analysis of GJB3 expression in HeLa.
Western Blotting Western Blot analysis of GJB3 expression in transfected 293T cell line by GJB3 monoclonal antibody, clone 3B4-1B3. Lanes Lane 1: GJB3 transfected lysate (31 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (55.44 kDa).
ELISA Detection limit for recombinant GST tagged GJB3 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3B4-1B3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC012918   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O75712 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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| General description: | This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein. (provided by RefSeq) |
| Immunogen: | GJB3 (AAH12918.1, 1 a.a. ~ 270 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDWKTLQALLSGVNKYSTAFGRIWL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |