Monoclonal Anti-GLB1 antibody produced in mouse
SIGMA/WH0002720M1 - clone 6E7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-galactosidase, beta 1
Product Type: Chemical
Western Blotting GLB1 monoclonal antibody, clone 6E7 Western Blot analysis of GLB1 expression in HeLa.
Western Blotting Western Blot analysis of GLB1 expression in transfected 293T cell line by GLB1 monoclonal antibody, clone 6E7. Lanes Lane 1: GLB1 transfected lysate (76.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
Immunoprecipitation Immunoprecipitation of GLB1 transfected lysate using anti-GLB1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with GLB1 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged GLB1 is 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6E7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_000404   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P16278 |
| General description: | This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the terminal beta-galactose from ganglioside substrates and other glycoconjugates. Defects in this gene are the cause of GM1-gangliosidosis and Morquio B syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | GLB1 (NP_000395, 578 a.a. ~ 677 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence KGQVWINGFNLGRYWPARGPQLTLF |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |