Monoclonal Anti-GRB7 antibody produced in mouse
SIGMA/WH0002886M3 - clone 3C12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-growth factor receptor-bound protein 7
MDL Number: MFCD01633405
Product Type: Chemical
Western Blotting Western Blot analysis of GRB7 expression in transfected 293T cell line by GRB7 monoclonal antibody, clone 3C12. Lanes Lane 1: GRB7 transfected lysate (59.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
Immunoprecipitation Immunoprecipitation of GRB7 transfected lysate using anti-GRB7 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with GRB7 rabbit polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3C12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC006535   |
| isotype | IgG2bλ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q14451 |
| General description: | The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Alternative splicing results in multiple transcript variants encoding different isoforms, although the full-length natures of only two of the variants have been determined to date. (provided by RefSeq) |
| Immunogen: | GRB7 (AAH06535, 37 a.a. ~ 136 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence EEVKRSQPLLIPTTGRKLREEERRA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |