Monoclonal Anti-H3F3B antibody produced in mouse
SIGMA/WH0003021M1 - clone 2D7-H1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-H3 histone, family 3B (H3.3B); Anti-H3.3B
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to H3F3B on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to H3F3B on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of H3F3B expression in transfected 293T cell line by H3F3B monoclonal antibody, clone 2D7-H1. Lanes Lane 1: H3F3B transfected lysate (15 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (40.7 kDa).
ELISA Detection limit for recombinant GST tagged H3F3B is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2D7-H1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC017558   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is poyadenylated, unlike most histone genes. The protein encoded is a member of the histone H3 family. (provided by RefSeq) |
| Immunogen: | H3F3B (AAH17558, 1 a.a. ~ 136 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MARTKQTARKSTGGKAPRKQLATKA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |