Monoclonal Anti-HAL antibody produced in mouse
SIGMA/WH0003034M4 - clone 4F2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HIS; Anti-HSTD; Anti-histidine ammonia-lyase
MDL Number: MFCD02096540
Product Type: Chemical
Western Blotting Western Blot analysis of HAL expression in transfected 293T cell line by HAL monoclonal antibody, clone 4F2. Lanes Lane 1: HAL transfected lysate (72.698 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.11 kDa).
Immunoprecipitation Immunoprecipitation of HAL transfected lysate using anti-HAL monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with HAL rabbit polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_002108   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P42357 |
| General description: | Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids (provided by RefSeq) |
| Immunogen: | HAL (NP_002099, 558 a.a. ~ 657 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence LAACQGIEFLRPLKTTTPLEKVYDL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |