Monoclonal Anti-HDAC1 antibody produced in mouse
SIGMA/WH0003065M2 - clone 3E1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DKFZp686H12203; Anti-GON10; Anti-HD1; Anti-RPD3; Anti-RPD3L1; Anti-histone deacetylase 1
MDL Number: MFCD01633420
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting HDAC1 monoclonal antibody, clone 3E1. Western Blot analysis of HDAC1 expression in NIH/3T3.
Western Blotting HDAC1 monoclonal antibody, clone 3E1 Western Blot analysis of HDAC1 expression in HeLa S3 NE.
Western Blotting HDAC1 monoclonal antibody, clone 3E1. Western Blot analysis of HDAC1 expression in PC-12.
Western Blotting HDAC1 monoclonal antibody, clone 3E1. Western Blot analysis of HDAC1 expression in Raw 264.7.
Western Blotting QC Western Blot detection against Immunogen (78.76 kDa).
ELISA Detection limit for recombinant GST tagged HDAC1 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3E1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC000301   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q13547 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. (provided by RefSeq) |
| Immunogen: | HDAC1 (AAH00301, 1 a.a. ~ 482 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAQTQGTRRKVCYYYDGDVGNYYYG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |