Monoclonal Anti-HFE antibody produced in mouse
SIGMA/WH0003077M1 - clone 1G12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HFE1; Anti-HH; Anti-HLAH; Anti-MGC103790; Anti-dJ221C16.10.1; Anti-hemochromatosis
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to HFE on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of HFE expression in transfected 293T cell line by HFE monoclonal antibody, clone 1G12. Lanes Lane 1: HFE transfected lysate (40.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting HFE monoclonal antibody, clone 1G12. Western Blot analysis of HFE expression in A-431.
Western Blotting QC Western Blot detection against Immunogen (35.75 kDa).
ELISA Detection limit for recombinant GST tagged HFE is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1G12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_000410   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q30201 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined. (provided by RefSeq) |
| Immunogen: | HFE (NP_000401, 115 a.a. ~ 205 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence SHTLQVILGCEMQEDNSTEGYWKYG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |