Monoclonal Anti-HMGB1 antibody produced in mouse
SIGMA/WH0003146M8 - clone 2F6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DKFZp686A04236; Anti-HMG1; Anti-HMG3; Anti-SBP1; Anti-high-mobility group box 1
MDL Number: MFCD02263062
Product Type: Chemical
antibody form | purified immunoglobulin |
antibody product type | primary antibodies |
biological source | mouse |
clone | 2F6, monoclonal |
conjugate | unconjugated |
form | buffered aqueous solution |
GenBank® accession no. | NM_002128 |
isotype | IgG2aκ |
Quality Level | 100 |
shipped in | dry ice |
species reactivity | human |
storage temp. | −20°C |
technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
indirect ELISA: suitable | |
indirect immunofluorescence: suitable | |
western blot: 1-5 μg/mL | |
UniProt accession no. | P09429 |
Application: | Monoclonal Anti-HMGB1 antibody produced in mouse has been used in the detection of HMGB1 in pancreatic ductal adenocarcinogenic (PDAC) cell lines using fluorescent microscopy. and in western blotting. |
Biochem/physiol Actions: | High mobility group box 1 (HMGB1) functions to stabilize nucleosome and also regulates gene transcription. The phosphorylation of the nuclear localization signal in HMGB1 regulates its transport between cytoplasm and nucleus. It assists in nucleosome remodeling by eliciting chaperone functionality. Inflammation triggers high expression of HMGB1 and its inhibition may be useful for treating refractory M. pneumoniae pneumonia (RMPP). Elevated levels of HMGB1 is present in cardiovascular diseases. HMGB1 regulates autophagy in human liver cells in absence of oxygen followed reperfusion. High levels HMGB1 expression is seen in adenocarcinoma (AC), gall bladder and squamous cell/adenosquamous (SC/ASC) cancer. Polymorphisms in HMGB1 is present in oral squamous cell carcinoma (OSCC). |
Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
General description: | High mobility group box 1 (HMGB1) is a DNA binding protein, consisting of negatively charged amino acids in C-terminus and two DNA binding motifs, called BOX A and B. HMGB1 is mapped to human chromosome 13q12.3. Necrotic cells and neuritis express HMGB1. Monocytes and macrophages upon activation release HMGB1. |
Immunogen: | HMGB1 (NP_002119, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGKGDPKKPRGKMSSYAFFVQTCRE |
Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
Physical form: | Solution in phosphate buffered saline, pH 7.4 |
RIDADR | NONH for all modes of transport |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352203 |