Monoclonal Anti-FOXA1 antibody produced in mouse
SIGMA/WH0003169M1 - clone 2D7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HNF3A; Anti-MGC33105; Anti-TCF3A; Anti-forkhead box A1
MDL Number: MFCD09265359
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 μg/mL]
Western Blotting Western Blot analysis of FOXA1 expression in transfected 293T cell line by FOXA1 monoclonal antibody, clone 2D7. Lanes Lane 1: FOXA1 transfected lysate (49.1 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of FOXA1 over-expressed 293 cell line, cotransfected with FOXA1 Validated Chimera RNAi. Blot probed with FOXA1 monoclonal antibody, clone 2D7. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting FOXA1 monoclonal antibody, clone 2D7. Western Blot analysis of FOXA1 expression in HepG2.
Western Blotting QC Western Blot detection against Immunogen (37.4 kDa).
ELISA Detection limit for recombinant GST tagged FOXA1 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2D7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_004496   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P55317 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. (provided by RefSeq) |
| Immunogen: | FOXA1 (NP_004487, 367 a.a. ~ 472 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence LASVPASHPAHGLAPHESQLHLKGD |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |