Monoclonal Anti-HPX antibody produced in mouse
SIGMA/WH0003263M1 - clone 3A9-1A9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-hemopexin
MDL Number: MFCD01324486
Product Type: Chemical
Western Blotting Western Blot analysis of HPX expression in transfected 293T cell line by HPX monoclonal antibody, clone 3A9-1A9. Lanes Lane 1: HPX transfected lysate (Predicted MW: 28.6 kDa). Lane 2: Non-transfected lysate.
Western Blotting HPX monoclonal antibody, clone 3A9-1A9. Western Blot analysis of HPX expression in human liver.
Enhanced Validation-RNAi Western blot analysis of HPX over-expressed 293 cell line, cotransfected with HPX Validated Chimera RNAi. Blot probed with HPX monoclonal antibody, clone 3A9-1A9. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (53.68 kDa).
Immunoprecipitation Immunoprecipitation of HPX transfected lysate using anti-HPX monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with HPX rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged HPX is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3A9-1A9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC005395   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P02790 |
| General description: | This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. (provided by RefSeq) |
| Immunogen: | HPX (AAH05395, 1 a.a. ~ 254 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MARVLGAPVALGLWSLCWSLAIATP |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |