Monoclonal Anti-HSPA1B antibody produced in mouse
SIGMA/WH0003304M2 - clone 3B7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HSP702; Anti-heat shock 70kDa protein 1B
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human testis. [antibody concentration 6 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to HSPA1B on HeLa cell. [antibody concentration 10 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between TP53 and HSPA1B. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-HSPA1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting HSPA1B monoclonal antibody, clone 3B7 Western Blot analysis of HSPA1B expression in IMR-32.
Western Blotting HSPA1B monoclonal antibody, clone 3B7. Western Blot analysis of HSPA1B expression in Raw 264.7.
Western Blotting HSPA1B monoclonal antibody, clone 3B7. Western Blot analysis of HSPA1B expression in PC-12.
Western Blotting HSPA1B monoclonal antibody, clone 3B7. Western Blot analysis of HSPA1B expression in NIH/3T3.
Western Blotting QC Western Blot detection against Immunogen (34.65 kDa).
ELISA Detection limit for recombinant GST tagged HSPA1B is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3B7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_005345   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P0DMV9 |
| General description: | This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. (provided by RefSeq) |
| Immunogen: | HSPA1B (NP_005336, 531 a.a. ~ 611 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence VQRERVSAKNALESYAFNMKSAVED |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |