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Monoclonal Anti-HSPE1 antibody produced in mouse

SIGMA/WH0003336M1 - clone 4C11-B11, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-CPN10; Anti-GROES; Anti-HSP10; Anti-heat shock 10kDa protein 1 (chaperonin 10)

MDL Number: MFCD00282265
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-WH0003336M1-100UG 100 µg
$524.00
1/EA
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Immunofluorescence Immunofluorescence of monoclonal antibody to HSPE1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting HSPE1 monoclonal antibody, clone 4C11-B11 Western Blot analysis of HSPE1 expression in COLO 320 HSR.
Western Blotting Western Blot analysis of HSPE1 expression in transfected 293T cell line by HSPE1 monoclonal antibody, clone 4C11-B11. Lanes Lane 1: HSPE1 transfected lysate (10.9 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.96 kDa).
ELISA Detection limit for recombinant GST tagged HSPE1 is approximately 0.1 ng/mL as a capture antibody.

 

antibody form purified immunoglobulin
antibody product type primary antibodies
biological source mouse
clone 4C11-B11, monoclonal
conjugate unconjugated
form buffered aqueous solution
GenBank® accession no. BC023518 
isotype IgG1κ
Quality Level 100 
shipped in dry ice
species reactivity human
storage temp. −20°C
technique(s) indirect ELISA: suitable
  indirect immunofluorescence: suitable
  western blot: 1-5 μg/mL
UniProt accession no. P61604 
Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description: This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. (provided by RefSeq)
Immunogen: HSPE1 (AAH23518, 1 a.a. ~ 102 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
MAGQAFRKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQATVVAVGSGSKGKGGEIQPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDGDILGKYVD
Legal Information: GenBank is a registered trademark of United States Department of Health and Human Services
Physical form: Solution in phosphate buffered saline, pH 7.4
RIDADR NONH for all modes of transport
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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