Monoclonal Anti-IMPDH2 antibody produced in mouse
SIGMA/WH0003615M1 - clone 1E12-B6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-IMP (inosine monophosphate) dehydrogenase 2; Anti-IMPD2
Product Type: Chemical
Western Blotting Western Blot analysis of IMPDH2 expression in transfected 293T cell line by IMPDH2 monoclonal antibody, clone 1E12-B6. Lanes Lane 1: IMPDH2 transfected lysate (55.8 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of IMPDH2 over-expressed 293 cell line, cotransfected with IMPDH2 Validated Chimera RNAi. Blot probed with IMPDH2 monoclonal antibody, clone 1E12-B6. GAPDH (36.1 kDa) used as specificity and loading control.
Immunoprecipitation Immunoprecipitation of IMPDH2 transfected lysate using anti-IMPDH2 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with IMPDH2 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged IMPDH2 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1E12-B6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC012840   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P12268 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5′-monophosphate into xanthine-5′-monophosphate |
| Immunogen: | IMPDH2 (AAH12840, 1 a.a. ~ 514 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MADYLISGGTSYVPDDGLTAQQLFN |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |