Monoclonal Anti-CD82 antibody produced in mouse
SIGMA/WH0003732M1 - clone 4F2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-4F9; Anti-C33; Anti-CD82 antigen; Anti-GR15; Anti-IA4; Anti-KAI1; Anti-R2; Anti-SAR2; Anti-ST6; Anti-TSPAN27
MDL Number: MFCD03096711
Product Type: Chemical
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between CD19 and CD82. HeLa cells were stained with anti-CD19 rabbit purified polyclonal 1:1200 and anti-CD82 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
ELISA Detection limit for recombinant GST tagged CD82 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC000726   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| proximity ligation assay: suitable | |
| UniProt accession no. | P27701 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | CD82 (AAH00726, 1 a.a. ~ 267 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGSACIKVTKYFLFLFNLIFFILGA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |