Monoclonal Anti-KPNA5 antibody produced in mouse
SIGMA/WH0003841M1 - clone 1D2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-IPOA6; Anti-SRP6; Anti-karyopherin alpha 5 (importin alpha 6)
Product Type: Chemical
Western Blotting Western Blot analysis of KPNA5 expression in transfected 293T cell line by KPNA5 monoclonal antibody, clone 1D2. Lanes Lane 1: KPNA5 transfected lysate (60.7 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of KPNA5 over-expressed 293 cell line, cotransfected with KPNA5 Validated Chimera RNAi. Blot probed with KPNA5 monoclonal antibody, clone 1D2. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting KPNA5 monoclonal antibody, clone 1D2 Western Blot analysis of KPNA5 expression in HepG2.
Western Blotting QC Western Blot detection against Immunogen (85.03 kDa).
ELISA Detection limit for recombinant GST tagged KPNA5 is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC047409   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O15131 |
| General description: | The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus. (provided by RefSeq) |
| Immunogen: | KPNA5 (AAH47409.1, 1 a.a. ~ 539 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDAMASPGKDNYRMKSYKNKALNPQ |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |