Monoclonal Anti-KRAS antibody produced in mouse
SIGMA/WH0003845M1 - clone 3B10-2F2, purified immunoglobulin, buffered aqueous solution
Synonym: KRAS Antibody - Monoclonal Anti-KRAS antibody produced in mouse; Kras Antibody; Anti-CKRAS; Anti-KIRAS; Anti-KRAS1; Anti-KRAS2; Anti-KRAS2A; Anti-KRAS2B; Anti-KRAS4A; Anti-KRAS4B; Anti-RASK2; Anti-v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
MDL Number: MFCD00239650
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to KRAS on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting KRAS monoclonal antibody, clone 3B10-2F2 Western Blot analysis of KRAS expression in HeLa.
Western Blotting Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody, clone 3B10-2F2. Lanes Lane 1: KRAS transfected lysate (21 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (46.42 kDa).
ELISA Detection limit for recombinant GST tagged KRAS is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | 3B10-2F2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC013572   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable | |
| immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P01116 |
| Application: | Monoclonal Anti-KRAS antibody produced in mouse has been used in immunoprecipitation, immunofluorescence, western blotting, indirect enzyme linked immunosorbent assay (ELISA). |
| Biochem/physiol Actions: | Kirsten rat sarcoma viral oncogene homologue (KRAS) is a key protein of the Ras signaling pathways. It facilitates the invasion and metastasis of tumors. Gain-of-function mutations in the gene leads to the development of variety of tumors, including pancreatic, biliary tract and colon tumors. This mutation is rarely observed in gastric cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Kirsten rat sarcoma viral oncogene homologue (KRAS) is an oncogene that is mapped to human chromosome 12p12.1. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. The gene codes for a member of the small GTPase superfamily. |
| Immunogen: | KRAS (AAH13572, 1 a.a. ~ 188 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MTEYKLVVVGAGGVGKSALTIQLIQ |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |