Monoclonal Anti-LCK antibody produced in mouse
SIGMA/WH0003932M1 - clone 3F7-F5, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-
MDL Number: MFCD00239720
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of LCK over-expressed 293 cell line, cotransfected with LCK Validated Chimera RNAi. Blot probed with LCK monoclonal antibody (M01) clone 3F7-F5. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of LCK expression in transfected 293T cell line by LCK monoclonal antibody, clone 3F7-F5. Lanes Lane 1: LCK transfected lysate (59.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting LCK monoclonal antibody, clone 3F7-F5 Western Blot analysis of LCK expression in Jurkat.
Western Blotting QC Western Blot detection against Immunogen (84.7 kDa).
Immunoprecipitation Immunoprecipitation of LCK transfected lysate using anti-LCK monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with LCK rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged LCK is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3F7-F5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC013200   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P06239 |
| General description: | This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containin |
| Immunogen: | LCK (AAH13200.1, 1 a.a. ~ 539 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGCGCSSHPEDDWMENIDVCENCHY |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |