Monoclonal Anti-LIMK2 antibody produced in mouse
SIGMA/WH0003985M1 - clone 2H2-E11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-LIM domain kinase 2
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to LIMK2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of LIMK2 expression in transfected 293T cell line by LIMK2 monoclonal antibody, clone 2H2-E11. Lanes Lane 1: LIMK2 transfected lysate (72.232 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (101.0899 kDa).
ELISA Detection limit for recombinant GST tagged LIMK2 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2H2-E11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC013051   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P53671 |
| General description: | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | LIMK2 (AAH13051.1, 1 a.a. ~ 686 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGSYLSVPAYFTSRDLFRCSECQDS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |