Monoclonal Anti-LIPA antibody produced in mouse
SIGMA/WH0003988M1 - clone 1F9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CESD; Anti-LAL; Anti-lipase A, lysosomal acid, cholesterol esterase (Wolman disease)
Product Type: Chemical
Western Blotting Western Blot analysis of LIPA expression in transfected 293T cell line by LIPA monoclonal antibody, clone 1F9. Lanes Lane 1: LIPA transfected lysate (45 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (69.63 kDa).
Immunoprecipitation Immunoprecipitation of LIPA transfected lysate using anti-LIPA monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with LIPA rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged LIPA is approximately 10 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1F9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC012287   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P38571 |
| General description: | This gene encodes lipase A, the lysosomal acid lipase (also known as cholesterol ester hydrolase). This enzyme functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides. Mutations in this gene can result in Wolman disease and cholesteryl ester storage disease. Alternatively spliced transcript variants encoding the same protein have been found for this gene. (provided by RefSeq) |
| Immunogen: | LIPA (AAH12287, 1 a.a. ~ 399 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MKMRFLGLVVCLVLWPLHSEGSGGK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |