Monoclonal Anti-SH2D1A antibody produced in mouse
SIGMA/WH0004068M1 - clone 1C9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DSHP; Anti-EBVS; Anti-IMD5; Anti-LYP; Anti-MTCP1; Anti-SAP; Anti-SH2 domain protein 1A, Duncan’s disease (lymphoproliferative syndrome); Anti-XLP; Anti-XLPD
Product Type: Chemical
Western Blotting SH2D1A monoclonal antibody, clone 1C9 Western Blot analysis of SH2D1A expression in Jurkat.
Western Blotting Western Blot analysis of SH2D1A expression in transfected 293T cell line by SH2D1A monoclonal antibody, clone 1C9. Lanes Lane 1: SH2D1A transfected lysate (14.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (39.82 kDa).
ELISA Detection limit for recombinant GST tagged SH2D1A is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1C9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC020732   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O60880 |
| General description: | This gene encodes a protein that plays a major role in the bidirectional stimulation of T and B cells. This protein contains an SH2 domain and a short tail. It associates with the signaling lymphocyte-activation molecule, thereby acting as an inhibitor of this transmembrane protein by blocking the recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to its docking site. This protein can also bind to other related surface molecules that are expressed on activated T, B and NK cells, thereby modifying signal transduction pathways in these cells. Mutations in this gene cause lymphoproliferative syndrome X-linked type 1 or Duncan disease, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus, with symptoms including severe mononucleosis and malignant lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | SH2D1A (AAH20732, 1 a.a. ~ 128 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDAVAVYHGKISRETGEKLLLATGL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |