Monoclonal Anti-MARCKS antibody produced in mouse
SIGMA/WH0004082M6 - clone 2C2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-80KL; Anti-FLJ14368; Anti-MACS; Anti-MRACKS; Anti-PKCSL; Anti-PRKCSL; Anti-myristoylated alanine-rich protein kinase C substrate
MDL Number: MFCD01633619
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to MARCKS on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to MARCKS on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting MARCKS monoclonal antibody, clone 2C2. Western Blot analysis of MARCKS expression in Raw 264.7. (The predicted M.W. of MARCKS is 29 to 39 kDa, but it seems the actual M.W. in vivo is between 68 to 90 kDa in different species. http://www.pnas.org/content/88/6/2505.full.pdf , http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=177061)
Western Blotting QC Western Blot detection against Immunogen (32.78 kDa).
ELISA Detection limit for recombinant GST tagged MARCKS is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2C2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_002356   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P26645 |
| Application: | Monoclonal Anti-MARCKS antibody produced in mouse has been used in immunohistochemistry (1:400). |
| Biochem/physiol Actions: | Myristoylated alanine-rich C-kinase substrate (MARCKS) is involved in mediating brain plasticity, inflammatory response, embryonic development, and regeneration processes. It also plays a key role in cell cycle regulation and transmembrane transport. Various studies have implicated the association of MARCKS with the pathophysiology of glioblastoma, cholangiocarcinoma, melanoma, and many more tumor types. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Myristoylated alanine-rich C-kinase substrate (MARCKS) contains an N-terminal domain (ND), effector domain (ED), and myristoylated MH2 domain. The MARCKS gene is mapped to human chromosome 6q21. |
| Immunogen: | MARCKS (NP_002347, 2 a.a. ~ 65 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence GAQFSKTAAKGEAAAERPGEAAVAS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |