Monoclonal Anti-SMAD3 antibody produced in mouse
SIGMA/WH0004088M5 - clone 4D5, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DKFZP586N0721; Anti-DKFZp686J10186; Anti-HSPC193; Anti-HsT17436; Anti-JV152; Anti-MADH3; Anti-MGC60396; Anti-SMAD, mothers against DPP homolog 3 (Drosophila); Anti-Smad 3
MDL Number: MFCD02097405
Product Type: Chemical
Western Blotting SMAD3 monoclonal antibody, clone 4D5. Western Blot analysis of SMAD3 expression in Jurkat.
Western Blotting SMAD3 monoclonal antibody, clone 4D5 Western Blot analysis of SMAD3 expression in PC-12.
ELISA Detection limit for recombinant GST tagged SMAD3 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4D5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_005902   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P84022 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene ′mothers against decapentaplegic′ (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. (provided by RefSeq) |
| Immunogen: | SMAD3 (NP_005893, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSSILPFTPPIVKRLLGWKKGEQNG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |