Monoclonal Anti-MAX antibody produced in mouse
SIGMA/WH0004149M1 - clone 4E10-1A9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MGC10775; Anti-MGC11225; Anti-MGC18164; Anti-MGC34679; Anti-MGC36767; Anti-MYC associated factor X; Anti-orf1
MDL Number: MFCD01633623
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to MAX on HeLa cell. [antibody concentration 10 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between MAPK14 and MAX. HeLa cells were stained with anti-MAPK14 rabbit purified polyclonal 1:1200 and anti-MAX mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting QC Western Blot detection against Immunogen (42.35 kDa).
ELISA Detection limit for recombinant GST tagged MAX is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4E10-1A9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC003525   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P61244 |
| Biochem/physiol Actions: | Myc-associated factor X (MAX) interacts with N-, L-, and c-Myc proteins both in vitro and in vivo. MAX plays a vital role in the regulation of the MYC/MAX/ MAX dimerization protein (MXD) network of basic helix-loop-helix leucine zipper (bHLHZip) transcription factors implicated in cell proliferation, differentiation, and apoptosis. Mutation of the gene leads to pheochromocytomas and paragangliomas (PPGLs). Heterodimer complex of c-Myc and Max proteins stimulates the transcription at target promoters by binding hexameric DNA consensus sequences “CANNTG” (E-boxes). Apart from this, Max hinders MYC-dependent cell transformation by transcriptional repression of the same E-boxes by associating with various repressors such as MXD, MAX network transcriptional repressor (MNT), and MAX gene-associated protein (MGA). Gene transcription activated by MYC–MAX heterodimers can be repressed by Max homodimers by decreasing the level of free MAX monomers and by binding to E-box DNA sequences. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown. (provided by RefSeq) |
| Immunogen: | MAX (AAH03525, 1 a.a. ~ 151 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSDNDDIEVESDADKRAHHNALERK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |