Monoclonal Anti-MCM3 antibody produced in mouse
SIGMA/WH0004172M1 - clone 4F7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HCC5; Anti-MCM3 minichromosome maintenance deficient 3 (S. cerevisiae); Anti-MGC1157; Anti-P1.h; Anti-P1MCM3; Anti-RLFB
MDL Number: MFCD02263436
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting MCM3 monoclonal antibody, clone 4F7 Western Blot analysis of MCM3 expression in COLO 320 HSR.
Western Blotting MCM3 monoclonal antibody, clone 4F7. Western Blot analysis of MCM3 expression in Raw 264.7.
Western Blotting MCM3 monoclonal antibody, clone 4F7. Western Blot analysis of MCM3 expression in Daoy.
Western Blotting MCM3 monoclonal antibody, clone 4F7. Western Blot analysis of MCM3 expression in A-431.
Western Blotting MCM3 monoclonal antibody, clone 4F7. Western Blot analysis of MCM3 expression in HeLa.
Western Blotting MCM3 monoclonal antibody, clone 4F7. Western Blot analysis of MCM3 expression in K-562.
Western Blotting MCM3 monoclonal antibody, clone 4F7. Western Blot analysis of MCM3 expression in human colon.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
ELISA Detection limit for recombinant GST tagged MCM3 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_002388   |
| isotype | IgG2aλ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P25206 |
| General description: | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. (provided by RefSeq) |
| Immunogen: | MCM3 (NP_002379, 699 a.a. ~ 808 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence AKDGDSYDPYDFSDTEEEMPQVHTP |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |