Monoclonal Anti-ME1 antibody produced in mouse
SIGMA/WH0004199M3 - clone 3H5, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HUMNDME; Anti-MES; Anti-malic enzyme 1, NADP(+)-dependent, cytosolic
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to ME1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.5 μg/mL]
Western Blotting ME1 monoclonal antibody, clone 3H5. Western Blot analysis of ME1 expression in HepG2.
Western Blotting Western Blot analysis of ME1 expression in transfected 293T cell line by ME1 monoclonal antibody, clone 3H5. Lanes Lane 1: ME1 transfected lysate (64.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (88.66 kDa).
ELISA Detection limit for recombinant GST tagged ME1 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3H5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC025246   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P06801 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. (provided by RefSeq) |
| Immunogen: | ME1 (AAH25246, 1 a.a. ~ 572 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MEPEAPRRRHTHQRGYLLTRNPHLN |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |