Monoclonal Anti-MEIS2 antibody produced in mouse
SIGMA/WH0004212M1 - clone 1H4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HsT18361; Anti-MGC2820; Anti-MRG1; Anti-Meis1, myeloid ecotropic viral integration site 1 homolog 2 (mouse)
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to MEIS2 on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 1 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to MEIS2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting MEIS2 monoclonal antibody, clone 1H4 Western Blot analysis of MEIS2 expression in HeLa.
Western Blotting MEIS2 monoclonal antibody, clone 1H4. Western Blot analysis of MEIS2 expression in K-562.
Western Blotting Western Blot analysis of MEIS2 expression in transfected 293T cell line by MEIS2 monoclonal antibody, clone 1H4. Lanes Lane 1: MEIS2 transfected lysate (51.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (67.65 kDa).
ELISA Detection limit for recombinant GST tagged MEIS2 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1H4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC001516   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O14770 |
| Biochem/physiol Actions: | Myeloid ecotropic insertion site 2 (MEIS2) plays a vital role in proximal-distal limb patterning, skeletal muscle differentiation, and the development of hindbrain, lens and retina. It is also involved in the regulation of cell proliferation and differentiation during development. Mutation in the gene leads to cleft palate and cardiac septal defects as well as intellectual disability. MEIS2 is overexpressed in human neuroblastoma cell lines and is essential for neuroblastoma cell survival and proliferation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a homeobox protein belonging to the TALE (′three amino acid loop extension′) family of homeodomain-containing proteins. TALE homeobox proteins are highly conserved transcription regulators, and several members have been shown to be essential contributors to developmental programs. Multiple transcript variants encoding distinct isoforms have been described for this gene. (provided by RefSeq) |
| Immunogen: | MEIS2 (AAH01516, 1 a.a. ~ 381 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDGVGVPASMYGDPHAPRPIPPVHH |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |