Monoclonal Anti-MLH1 antibody produced in mouse
SIGMA/WH0004292M2 - clone M1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-COCA2; Anti-FCC2; Anti-HNPCC; Anti-HNPCC2; Anti-MGC5172; Anti-hMLH1; Anti-mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
MDL Number: MFCD00282360
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to MLH1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to MLH1 on HeLa cell. [antibody concentration 10 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between MSH2 and MLH1. HeLa cells were stained with anti-MSH2 rabbit purified polyclonal 1:1200 and anti-MLH1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting MLH1 monoclonal antibody, clone M1. Western Blot analysis of MLH1 expression in K-562.
Western Blotting QC Western Blot detection against Immunogen (108.9 kDa).
ELISA Detection limit for recombinant GST tagged MLH1 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | M1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC006850   |
| isotype | IgGκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P40692 |
| Application: | Monoclonal Anti-MLH1 antibody produced in mouse has been used for immunocytochemistry and Western blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | MutL homolog 1 (MLH1) is a nucleoprotein and a major component of mismatch repair system. The MLH1 gene is localized on human chromosome 3p21 and is made up of 19 exons. The protein has a molecular weight of 80kDa. MLH1 promotes colorectal carcinogenesis. |
| Immunogen: | MLH1 (AAH06850, 1 a.a. ~ 756 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSFVAGVIRRLDETVVNRIAAGEVI |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |