Monoclonal Anti-MPP3 antibody produced in mouse
SIGMA/WH0004356M1 - clone 4D7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DLG3; Anti-membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3)
Product Type: Chemical
Western Blotting Western Blot analysis of MPP3 expression in transfected 293T cell line by MPP3 monoclonal antibody, clone 4D7. Lanes Lane 1: MPP3 transfected lysate (66.2 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of MPP3 over-expressed 293 cell line, cotransfected with MPP3 Validated Chimera RNAi. Blot probed with MPP3 monoclonal antibody, clone 4D7. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (90.09 kDa).
ELISA Detection limit for recombinant GST tagged MPP3 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4D7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC056865   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q13368 |
| Biochem/physiol Actions: | MPP3 (Membrane protein, palmitoylated 3, MAGUK p55 subfamily member 3) plays an important role in the maintenance of epithelial structure through cell-cell interactions. The N-terminal domain of MPP3 directly binds to the cytoplasmic domain of CADM1 (Cell adhesion molecule 1) and forms a complex, which further connects it to the PI3K (phosphoinositide 3-kinase) for its activation. The activation of PI3K is highly required to identify the actin cytoskeleton and form epithelial cell structure. A study reports that MPP3 in conjugation with TSLC1 (Tumor suppressor in lung cancer 1) shows tumor suppressive property in human lung cancer cells and any change in this interactions might lead to malignancy and tumor growth. |
| Immunogen: | MPP3 (AAH56865, 1 a.a. ~ 585 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MPVLSEDSGLHETLALLTSQLRPDS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |