Monoclonal Anti-MUTYH antibody produced in mouse
SIGMA/WH0004595M1 - clone 4D10, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MYH; Anti-MYHbeta; Anti-hMYH; Anti-mutY homolog (E. coli)
Product Type: Chemical
Western Blotting Western Blot analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody, clone 4D10. Lanes Lane 1: MUTYH transfected lysate (59.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting MUTYH monoclonal antibody, clone 4D10 Western Blot analysis of MUTYH expression in HeLa S3 NE.
Enhanced Validation-RNAi Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi. Blot probed with MUTYH monoclonal antibody, clone 4D10. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
Immunoprecipitation Immunoprecipitation of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with MUTYH rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged MUTYH is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4D10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC003178   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9UIF7 |
| General description: | This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | MUTYH (AAH03178, 436 a.a. ~ 535 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence KLTYQVYGLALEGQTPVTTVPPGAR |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |