Monoclonal Anti-MYOC antibody produced in mouse
SIGMA/WH0004653M1 - clone 4F8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-GLC1A; Anti-GPOA; Anti-JOAG; Anti-JOAG1; Anti-TIGR; Anti-myocilin; Anti-myocilin, trabecular meshwork inducible glucocorticoid response
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to MYOC on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 μg/mL]
Western Blotting Western Blot analysis of MYOC expression in transfected 293T cell line by MYOC monoclonal antibody, clone 4F8. Lanes Lane 1: MYOC transfected lysate (57 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of MYOC over-expressed 293 cell line, cotransfected with MYOC Validated Chimera RNAi. Blot probed with MYOC monoclonal antibody, clone 4F8. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
Immunoprecipitation Immunoprecipitation of MYOC transfected lysate using anti-MYOC monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with MYOC rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged MYOC is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC029261   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q99972 |
| General description: | MYOC encodes the protein myocilin, which is believed to have a role in cytoskeletal function. MYOC is expressed in many occular tissues, including the trabecular meshwork, and was revealed to be the trabecular meshwork glucocorticoid-inducible response protein (TIGR). The trabecular meshwork is a specialized eye tissue essential in regulating intraocular pressure, and mutations in MYOC have been identified as the cause of hereditary juvenile-onset open-angle glaucoma. (provided by RefSeq) |
| Immunogen: | MYOC (AAH29261, 36 a.a. ~ 135 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence QLRKANDQSGRCQYTFSVASPNESS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |