Monoclonal Anti-MYOG antibody produced in mouse
SIGMA/WH0004656M1 - clone 2B7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MYF4; Anti-MYOGENIN; Anti-myogenin (myogenic factor 4)
MDL Number: MFCD01322371
Product Type: Chemical
Western Blotting Western Blot analysis of MYOG expression in transfected 293T cell line by MYOG monoclonal antibody, clone 2B7. Lanes Lane 1: MYOG transfected lysate (25 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of MYOG over-expressed 293 cell line, cotransfected with MYOG Validated Chimera RNAi. Blot probed with MYOG monoclonal antibody, clone 2B7. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (50.38 kDa).
ELISA Detection limit for recombinant GST tagged MYOG is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2B7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC053899   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P15173 |
| Biochem/physiol Actions: | MYOG (myogenin) helps in the determination of skeletal muscle. It promotes myoblast differentiation. Myog, along with Myod is involved in a feed-forward circuit, which may help in patterning the muscle gene expression. |
| General description: | Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. (provided by RefSeq) MYOG (myogenin) is one of the muscle regulatory factors that belong to the b-HLH transcription factors family. This gene is located on human chromosome 1q32.1. |
| Immunogen: | MYOG (AAH53899, 1 a.a. ~ 224 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MELYETSPYFYQEPRFYDGENYLPV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |