Monoclonal Anti-NDUFV1 antibody produced in mouse
SIGMA/WH0004723M1 - clone 4A7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa; Anti-UQOR1
Product Type: Chemical
Western Blotting NDUFV1 monoclonal antibody, clone 4A7. Western Blot analysis of NDUFV1 expression in PC-12.
Western Blotting Western Blot analysis of NDUFV1 expression in transfected 293T cell line by NDUFV1 monoclonal antibody, clone 4A7. Lanes Lane 1: NDUFV1 transfected lysate (50.8 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of NDUFV1 over-expressed 293 cell line, cotransfected with NDUFV1 Validated Chimera RNAi. Blot probed with NDUFV1 monoclonal antibody, clone 4A7. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged NDUFV1 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4A7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_007103   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P49821 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The NDUFV1 gene encodes the 51-kD subunit of complex I (NADH:ubiquinone oxidoreductase) of the mitochondrial respiratory chain.[supplied by OMIM |
| Immunogen: | NDUFV1 (NP_002466, 365 a.a. ~ 464 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence KAIARLIEFYKHESCGQCTPCREGV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |