Monoclonal Anti-NELL1 antibody produced in mouse
SIGMA/WH0004745M1 - clone 6A8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-IDH3GL; Anti-NEL-like 1 (chicken); Anti-NRP1
MDL Number: MFCD02097027
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to NELL1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of NELL1 expression in transfected 293T cell line by NELL1 monoclonal antibody, clone 6A8. Lanes Lane 1: NELL1 transfected lysate (89.607 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
Immunoprecipitation Immunoprecipitation of NELL1 transfected lysate using anti-NELL1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with NELL1 rabbit polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6A8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_006157   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q92832 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Two transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | NELL1 (NP_006148, 304 a.a. ~ 403 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence CRRMSCPPLNCSPDSLPVHIAGQCC |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |