Monoclonal Anti-NEK3 antibody produced in mouse
SIGMA/WH0004752M1 - clone 2F8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HSPK36; Anti-MGC29949; Anti-NIMA (never in mitosis gene a)-related kinase 3
MDL Number: MFCD00803884
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to NEK3 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of NEK3 expression in transfected 293T cell line by NEK3 monoclonal antibody, clone 2F8. Lanes Lane 1: NEK3 transfected lysate (57.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged NEK3 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2F8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC019916   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P51956 |
| General description: | This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene |
| Immunogen: | NEK3 (AAH19916, 406 a.a. ~ 506 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence QWLKETPDTLLNILKNADLSLAFQT |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |