Monoclonal Anti-NFKB1 antibody produced in mouse
SIGMA/WH0004790M1 - clone 2E6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DKFZp686C01211; Anti-EBP1; Anti-KBF1; Anti-MGC54151; Anti-NFKBp105; Anti-NFKBp50; Anti-NFkappaB; Anti-nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105)
MDL Number: MFCD00802846
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between IKBKB and NFKB1. HeLa cells were stained with anti-IKBKB rabbit purified polyclonal 1:1200 and anti-NFKB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Enhanced Validation-RNAi Western blot analysis of NFKB1 over-expressed 293 cell line, cotransfected with NFKB1 Validated Chimera RNAi. Blot probed with NFKB1 monoclonal antibody, clone 2E6. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody, clone 2E6. Lanes Lane 1: NFKB1 transfected lysate (105.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.73 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC051765   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P19838 |
| Biochem/physiol Actions: | NFKB1 (nuclear factor κB subunit 1) controls the maturation of NK (natural killer) cells and effector functions. This gene helps in the upregulation of intrauterine adhesion inflammatory factors, hence NFKB1 plays a major role in inflammatory diseases. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | NFKB1 (nuclear factor κB subunit 1) is a dimeric transcription factor. It belongs to the REL family. This gene is located on human chromosome 4q24. |
| Immunogen: | NFKB1 (AAH51765, 860 a.a. ~ 969 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDNYEVSGGTVRELVEALRQMGYTE |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |