Monoclonal Anti-NME1 antibody produced in mouse
SIGMA/WH0004830M2 - clone 1D7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-AWD; Anti-GAAD; Anti-NDPKA; Anti-NM23; Anti-NM23H1; Anti-non-metastatic cells 1, protein (NM23A) expressed in
MDL Number: MFCD01865222
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to NME1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody, clone 1D7. Lanes Lane 1: NME1 transfected lysate (19.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting NME1 monoclonal antibody, clone 1D7. Western Blot analysis of NME1 expression in different cell lines.
Western Blotting NME1 monoclonal antibody, clone 1D7. Western Blot analysis of NME1 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
Immunoprecipitation Immunoprecipitation of NME1 transfected lysate using anti-NME1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with NME1 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged NME1 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_000269   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P15531 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of ′A′ (encoded by this gene) and ′B′ (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. (provided by RefSeq) |
| Immunogen: | NME1 (NP_000260, 43 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence ASEDLLKEHYVDLKDRPFFAGLVKY |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |