Monoclonal Anti-NME2 antibody produced in mouse
SIGMA/WH0004831M1 - clone 4B7-3F12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-NDPKB; Anti-NM23B; Anti-NM23H2; Anti-non-metastatic cells 2, protein (NM23B) expressed in; Anti-puf
MDL Number: MFCD01865221
Product Type: Chemical
Western Blotting Western Blot analysis of NME2 expression in transfected 293T cell line by NME2 monoclonal antibody, clone 4B7-3F12. Lanes Lane 1: NME2 transfected lysate (17.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting NME2 monoclonal antibody, clone 4B7-3F12 Western Blot analysis of NME2 expression in Jurkat.
Enhanced Validation-RNAi Western blot analysis of NME2 over-expressed 293 cell line, cotransfected with NME2 Validated Chimera RNAi. Blot probed with NME2 monoclonal antibody, clone 4B7-3F12. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (42.46 kDa).
ELISA Detection limit for recombinant GST tagged NME2 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4B7-3F12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC002476   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P22392 |
| General description: | Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of ′A′ (encoded by NME1) and ′B′ (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product. (provided by RefSeq) |
| Immunogen: | NME2 (AAH02476, 1 a.a. ~ 152 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MANLERTFIAIKPDGVQRGLVGEII |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |