Monoclonal Anti-NPM1 antibody produced in mouse
SIGMA/WH0004869M1 - clone 3B2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-B23; Anti-NPM; Anti-nucleophosmin (nucleolar phosphoprotein B23, numatrin)
MDL Number: MFCD03455664
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Enhanced levels of double-strand DNA break repair proteins protect ovarian cancer cells against genotoxic stress-induced apoptosis. By: Kalra, R. S. & Bapat, S. A. in J Ovarian Res, 2013. PubMed ID: 24044516 Image collected and cropped by CiteAb from the following publication, (http://ovarianresearch.biomedcentral.com/articles/10.1186/1757-2215-6-66), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Enhanced levels of double-strand DNA break repair proteins protect ovarian cancer cells against genotoxic stress-induced apoptosis. By: Kalra, R. S. & Bapat, S. A. in J Ovarian Res, 2013. PubMed ID: 24044516 Image collected and cropped by CiteAb from the following publication, (http://ovarianresearch.biomedcentral.com/articles/10.1186/1757-2215-6-66), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Enhanced levels of double-strand DNA break repair proteins protect ovarian cancer cells against genotoxic stress-induced apoptosis. By: Kalra, R. S. & Bapat, S. A. in J Ovarian Res, 2013. PubMed ID: 24044516 Image collected and cropped by CiteAb from the following publication, (http://ovarianresearch.biomedcentral.com/articles/10.1186/1757-2215-6-66), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Immunoperoxidase of monoclonal antibody to NPM1 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. [antibody concentration 5 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to NPM1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting NPM1 monoclonal antibody, clone 3B2 Western Blot analysis of NPM1 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (58.08 kDa).
ELISA Detection limit for recombinant GST tagged NPM1 is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | 3B2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC002398   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | ELISA: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P06748 |
| Application: | Monoclonal Anti-NPM1 antibody produced in mouse has been used in immunoblotting. |
| Application: | The antibody may be used for ELISA, competitive ELISA, immunoblotting (37 kDa), immunoprecipitation, immunohistochemistry, immunocytochemistry (2% formaldehyde-acetone 1,2,5 or 10% formalin/methanol-1% NP-40 and microinjection (blocks the initiation of centrosome duplication). Reactivity has been observed with human, monkey, bovine, dog, hamster (weak), rat,kangaroo rat, and mouse B23. |
| Biochem/physiol Actions: | NPM1 (nucleophosmin 1) induces cell proliferation. It increases migration and invasion of cells. |
| Biochem/physiol Actions: | NPM1 (nucleophosmin 1) induces cell proliferation. It increases migration and invasion of cells. Mutations in NPM1 result in acute myeloid leukemia (AML). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF (MIM 600160). NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia (AML; MIM 601626) (Garzon et al., 2008 [PubMed 18308931]). This gene is located on human chromosome 5q35. |
| General description: | NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF (MIM 600160). NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia (AML; MIM 601626) (Garzon et al., 2008 [PubMed 18308931]).[supplied by OMIM] Nucleophosmin 1 (NPM1) is an important multifunctional protein mainly located in the nucleolus. This gene is located on human chromosome 5q35. |
| Immunogen: | NPM1 (AAH02398.1, 1 a.a. ~ 294 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MEDSMDMDMSPLRPQNYLFGCELKA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |